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Related Experiment Video

Updated: May 1, 2026

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

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Published on: December 3, 2012

Multiphoton microscopy applications in nanodermatology.

¹Dermatology Research Centre, School of Medicine, The University of Queensland, Princes Alexandra Hospital, Woolloongabba, Queensland, Australia. t.prow@uq.edu.au

Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology

|September 14, 2012

Summary

This summary is machine-generated.

Multiphoton microscopy tracks nanoparticles and cellular molecules using femtosecond lasers. This technique enables dye-free metabolic assessments and advances nanomedicine and nanotoxicology research.

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Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

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Area of Science:

Biomedical Optics
Nanotechnology
Molecular Imaging

Background:

Multiphoton microscopy is a powerful tool for biological imaging.
It allows for deep tissue penetration and reduced phototoxicity.
Current limitations include distinguishing nanoparticle signals from autofluorescence.

Purpose of the Study:

To utilize multiphoton microscopy for simultaneous tracking of nanoparticles and endogenous autofluorescent molecules.
To develop a dye-free method for assessing treatment-induced metabolic changes.
To enhance the capabilities of nanomedicine and nanotoxicology research.

Main Methods:

Employing femtosecond lasers for excitation of various nanoparticles (metal, quantum dots, upconverting).
Integrating time-correlated single-photon counting detectors for fluorescence lifetime imaging.
Utilizing fluorescence lifetime measurements for high-resolution, quantitative data acquisition.

Main Results:

Successful distinction between nanoparticle signals and endogenous fluorophores.
Demonstration of simultaneous nanoparticle and NAD(P)H imaging.
Quantitative data enabling dye-free assessment of metabolic alterations.

Conclusions:

Multiphoton microscopy with fluorescence lifetime imaging offers advanced capabilities for nanoparticle tracking and metabolic analysis.
This approach facilitates dye-free assessments crucial for nanomedicine and nanotoxicology.
The technology is poised for clinical imaging trials addressing key nanomedicine challenges.