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Related Experiment Videos

Apoprotein E biosynthesis in the cholesterol-fed guinea pig.

D M Driscoll1, T Mazzone, T Matsushima

  • 1Department of Pathology, University of Chicago, Illinois.

Arteriosclerosis (Dallas, Tex.)
|January 1, 1990
PubMed
Summary

High cholesterol diets, regardless of fat type, increase apoprotein E (apo E) mRNA in guinea pig livers. This suggests a regulatory mechanism for apo E production in response to dietary lipids.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nutritional Science

Background:

  • Dietary cholesterol and fat intake significantly impact lipid metabolism and lipoprotein synthesis.
  • Apoprotein E (apo E) plays a crucial role in lipoprotein clearance and lipid transport.
  • Understanding the regulation of apo E biosynthesis is vital for managing hypercholesterolemia.

Purpose of the Study:

  • To investigate the effect of high cholesterol diets, supplemented with either polyunsaturated (corn oil) or saturated (coconut oil) fat, on apoprotein E mRNA levels in guinea pig livers.
  • To determine the temporal relationship between dietary interventions and changes in hepatic apo E mRNA abundance.

Main Methods:

  • Guinea pigs were fed control chow, or diets containing 1% cholesterol plus 5% corn oil or 5% coconut oil for 12 weeks.
  • Hypercholesterolemia was induced and monitored.
  • Hepatic apoprotein E mRNA levels were quantified using cell-free translation assays and membrane hybridization.

Main Results:

  • Both experimental diets induced hypercholesterolemia, with the coconut oil diet leading to higher lipid levels.
  • Hepatic cholesterol and triglyceride content increased significantly in animals on experimental diets.
  • Apoprotein E mRNA levels were elevated (1.7-2.5 fold) in the livers of animals fed cholesterol with either corn oil or coconut oil, with variations in the onset of this increase.

Conclusions:

  • High cholesterol diets, irrespective of the type of accompanying fat (saturated or polyunsaturated), lead to increased abundance of apoprotein E mRNA in the liver.
  • These findings highlight a regulatory response in hepatic apo E gene expression influenced by dietary lipid composition.
  • The study discusses the link between elevated apo E mRNA and previously observed increases in apo E synthesis and circulating levels.

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