Diagnosis of Dopamine in Brain Neuro Cell Using a Nafion-immobilized Carbon Electrode

Nafion immobilized onto a graphite pencil carbon electrode (NPE) was used to a voltammetric assay of dopamine. The optimum analytical conditions of 150 Hz frequency, 150 mV amplitude, -2.0 V accumulation potential and 15 mV increment potential were obtained. The SW working range was attained to 0.0-80.0 ugL-1 and the CV range of 0.0-90 mgL-1. The statistic relative standard deviation of the 10.0mgL-1 dopamine was 0.041 (n = 20) at the optimum conditions. The analytical detection limit (S/N) was attained to 0.49 ugL-1 (2.62×10-9 M). The developed method was applied to diagnostic assay at the fish brain core and diluted nerve tissue within a pre-concentration time of 60 sec.