Related Experiment Video
Updated: May 18, 2026

Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.
Published on: April 5, 2018
Fusogenic metallosupramolecular brush vesicles
Lipeng He1, Shuai Bi, Hui Wang
1Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou City, Gansu Province, China.
Abstract:
The electrostatic combination of a cationic metallosupramolecular polyelectrolyte (Fe-MSP) with sulfonate-terminated polymers leads to the formation of metallosupramolecular brushes (MSBs). The resulting MSBs can self-assemble into vesicular structures in chloroform/methanol (v/v = 1:1) mixture solvents. The rigid-rod Fe-MSP chain has to bend for the formation of the vesicles, which accompanies the presence of a lateral tension and thus induces a spontaneous vesicle fusion with an hour-scale fusion time. For this much longer fusion process, the arrow-like protrusion, stalk-like intermediate, and hemifusion diaphragm are clearly observed by transmission electron microscopy. The complete fusion into larger vesicles significantly releases the lateral tension.
More Related Videos
Related Concept Videos
Fusion of Secretory Vesicles with the Plasma Membrane
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
Pinching-off of Coated Vesicles
Intralumenal Vesicles and Multivesicular Bodies
SNAREs and Membrane Fusion
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
Vesicular Tubular Clusters
With the help of motor proteins such...
COP Coated Vesicles

