Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gradient metapopulation microfluidic ecologies shape genetic and biofilm drivers of T4r phage resistance in E. coli.

NPJ biofilms and microbiomes·2026
Same author

Informational Memory Shapes Collective Behavior in Intelligent Swarms.

Physical review letters·2026
Same author

Liposome purification from micromolar protein background using diffusiophoretic trapping.

Nanoscale·2026
Same author

Protocol to model tumor hypoxia in vitro using real-time phosphorescence-based sensing of O<sub>2</sub> gradients generated by metastatic cancer cells.

STAR protocols·2025
Same author

DNA Nanostructures Characterized via Dual Nanopore Resensing.

ACS nano·2025
Same author

Single-molecule capture, release, and dynamical manipulation via reversible electrokinetic confinement (RECON).

Science advances·2025

Related Experiment Video

Updated: May 18, 2026

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

DNA confinement in nanochannels: physics and biological applications.

Walter Reisner1, Jonas N Pedersen, Robert H Austin

  • 1Physics Department, McGill University, Montreal QC, Canada. reisner@physics.mcgill.ca

Reports on Progress in Physics. Physical Society (Great Britain)
|September 15, 2012
PubMed
Summary

Scientists are using nanochannels to stretch long DNA molecules for direct genetic analysis. This method bypasses traditional sequencing steps, enabling single-cell genome studies in their natural state.

More Related Videos

Monitoring Protein Adsorption with Solid-state Nanopores
08:51

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

Generation and Control of Electrohydrodynamic Flows in Aqueous Electrolyte Solutions
08:41

Generation and Control of Electrohydrodynamic Flows in Aqueous Electrolyte Solutions

Published on: September 7, 2018

Related Experiment Videos

Last Updated: May 18, 2026

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

Monitoring Protein Adsorption with Solid-state Nanopores
08:51

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

Generation and Control of Electrohydrodynamic Flows in Aqueous Electrolyte Solutions
08:41

Generation and Control of Electrohydrodynamic Flows in Aqueous Electrolyte Solutions

Published on: September 7, 2018

Area of Science:

  • Molecular Biology
  • Biophysics
  • Genomics

Background:

  • Reading genetic information from DNA is crucial in biology.
  • Current sequencing technologies have limitations for single-cell, native genome analysis.
  • Direct readout from long single DNA molecules is a growing area of interest.

Purpose of the Study:

  • To review how physical confinement in nanochannels allows access to information in genomic-length single DNA molecules.
  • To discuss the physics of DNA nanochannel confinement and its applications in genomic mapping.

Main Methods:

  • Utilizing nanochannels to physically confine long single DNA molecules.
  • Exploiting DNA's self-avoidance interactions to achieve linear stretching within nanochannels.
  • Analyzing the fundamental physics of DNA confinement, including ionic strength effects.

Main Results:

  • DNA molecules stretch linearly when confined in nanochannels, enabling analysis.
  • Nanochannel confinement provides a method for genomic mapping of single DNA molecules.
  • This approach allows probing of single-molecule conformation across relevant physical length scales.

Conclusions:

  • Nanochannel confinement offers a powerful platform for direct, native analysis of single DNA molecules.
  • This technique facilitates single-cell, single-genome analysis by eliminating amplification steps.
  • The physics of DNA confinement in nanochannels is a fascinating area with significant biological applications.