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Molecular identification of enteroviruses associated with aseptic meningitis in children from India

Arvind Kumar1, Deepti Shukla, Rashmi Kumar

  • 1Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Raebareli Road, Lucknow, 226014 Uttar Pradesh, India.

Archives of Virology
|September 15, 2012
PubMed

Insights

This study identified enteroviruses in children with aseptic meningitis, finding echovirus 32 to be predominant. Seven enterovirus serotypes were identified, with echovirus 32 newly associated with an aseptic meningitis epidemic.

Area of Science:

  • Virology
  • Pediatrics
  • Epidemiology

Background:

  • Aseptic meningitis in children is a significant public health concern.
  • Enteroviruses are a common cause of viral meningitis.

Purpose of the Study:

  • To identify and characterize enteroviruses causing aseptic meningitis in children.
  • To determine the predominant enterovirus serotypes involved in these infections.

Main Methods:

  • Enterovirus RNA detection in cerebrospinal fluid (CSF) samples using RT-PCR.
  • Molecular typing and sequencing of the VP1 region for enterovirus identification.
  • Phylogenetic analysis of identified enterovirus strains.

Main Results:

  • Enterovirus RNA was detected in 45.5% of 112 CSF samples.
  • Echovirus 32 was the predominant serotype (n=20), followed by echovirus 11 (n=10).
  • Seven distinct enterovirus serotypes were identified, including a novel association of echovirus 32 with an aseptic meningitis epidemic.

Conclusions:

  • Enteroviruses are a major cause of aseptic meningitis in the studied pediatric population.
  • Echovirus 32 emerged as a significant etiological agent during the study period.
  • This study provides crucial epidemiological data on enterovirus serotypes causing meningitis in India.

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