Related Experiment Video
Updated: May 18, 2026

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
Published on: November 9, 2017
Cytokine analysis by immunoaffinity capillary electrophoresis.
Mark Mendonca1, Heather Kalish
1Micro Analytical Immunochemistry Unit, Biomedical Engineering and Physical Science Shared Resource, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
Immunoaffinity capillary electrophoresis (ICE) enables precise detection of proteins in biological samples. This method uses antibodies in a capillary to capture, label, and quantify target proteins like cytokines and chemokines.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Immunology
Background:
- Immunoaffinity capillary electrophoresis (ICE) is a valuable technique for analyzing complex biological fluids.
- Accurate quantification of specific proteins, such as cytokines and chemokines, is crucial in clinical diagnostics.
Purpose of the Study:
- To demonstrate the construction of an immunoaffinity capillary.
- To outline the utilization of ICE for capturing and quantifying target cytokines and chemokines from clinical samples.
Main Methods:
- Immobilization of antibodies onto capillary walls for target analyte capture.
- In situ labeling with fluorescent dyes for detection.
- Online elution and laser-induced fluorescence detection following electrophoretic separation.
Main Results:
- Successful construction of an immunoaffinity capillary.
- Demonstrated capability of ICE to capture and quantify target cytokines and chemokines.
- Validation of the method for use with clinical samples.
Conclusions:
- ICE is a robust method for sensitive protein quantification in biological matrices.
- The described protocol facilitates the application of ICE in clinical settings for biomarker analysis.
More Related Videos
Related Concept Videos
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Affinity Chromatography
Electrophoresis: Overview
There...
Capillary Electrophoresis: Instrumentation

