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Updated: May 18, 2026

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications
Published on: April 21, 2023
Detection of posttranslational modifications of replication protein A
Cathy S Hass1, Ran Chen, Marc S Wold
1Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Abstract:
Replication Protein A (RPA) is a single-strand DNA-binding protein that is found in all eukaryotes. RPA is subjected to multiple posttranslational modifications including serine- and threonine-phosphorylation, poly-ADP ribosylation, and SUMOylation. These modifications are believed to regulate RPA activity through modulating interactions with DNA and partner proteins. This article describes two methods used to detect posttranslational modified RPA: immunofluorescence and immmuoblotting.
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