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Updated: May 18, 2026

Isolation of Mouse Lung Dendritic Cells
Published on: November 22, 2011
Chicken dendritic cells and type II pneumocytes express a common intracellular epitope
1Department of Human Morphology and Developmental Biology, Semmelweis University, 1094 Budapest, Tüzoltó u. 58, Hungary.
A new monoclonal antibody, CVI-ChNL 74·3, specifically identifies chicken lung type II pneumocytes. This antibody offers a valuable tool for monitoring the health and status of these crucial surfactant-producing cells in avian lungs.
Area of Science:
- Immunology
- Pulmonary Biology
- Avian Medicine
Background:
- Dendritic cells play a role in immune responses.
- Type II pneumocytes in the lung are responsible for surfactant production.
- Specific markers for avian lung cells are needed for research.
Purpose of the Study:
- To characterize the specificity of the CVI-ChNL 74·3 monoclonal antibody.
- To evaluate its utility in identifying chicken lung type II pneumocytes.
- To establish a tool for monitoring avian pulmonary health.
Main Methods:
- Development and characterization of the CVI-ChNL 74·3 monoclonal antibody.
- Immunohistochemical analysis of chicken lung tissue.
- Cross-reactivity testing against various avian and mammalian cell types.
Main Results:
- The CVI-ChNL 74·3 monoclonal antibody specifically binds to dendritic cells and chicken lung type II pneumocytes.
- No cross-reactivity was observed with other avian or mammalian granular pneumocytes.
- The antibody effectively labels surfactant-producing cells in the chicken lung.
Conclusions:
- CVI-ChNL 74·3 is a highly specific marker for chicken lung type II pneumocytes.
- This monoclonal antibody serves as a valuable tool for studying avian lung physiology and pathology.
- It facilitates the monitoring of type II pneumocyte status in chickens.
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