Related Experiment Video
Updated: May 18, 2026
09:22
Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
Published on: November 26, 2013
[Construction and identification of small interfering RNA expression vector targeting ATF-2 gene]
Wei-wei Mao1, Peng Xiong, Feng Han
1Department of Laboratory Medicine, Faculty of Clinical Medicine, Jiujiang, China.
Summary
A novel eukaryotic expression vector targeting the activating transcription factor 2 (ATF-2) gene was successfully constructed. This vector inhibits HepG2 cell proliferation and induces apoptosis, offering potential therapeutic strategies.
Area of Science:
- Molecular Biology
- Gene Regulation
Context:
- Activating transcription factor 2 (ATF-2) plays a role in cellular processes.
- HepG2 cells are a human hepatocellular carcinoma cell line commonly used in research.
Purpose:
- To construct an RNA interference (RNAi) eukaryotic expression vector targeting the ATF-2 gene.
- To investigate the effects of ATF-2 gene silencing on HepG2 cell proliferation and apoptosis.
Summary:
- A recombinant plasmid, PBA-siATF-2, was successfully constructed and confirmed by DNA sequencing.
- Transfection of HepG2 cells with PBA-siATF-2 led to down-regulation of ATF-2 protein levels.
- This ATF-2 inhibition resulted in reduced HepG2 cell proliferation and increased apoptosis.
Impact:
- Successful construction of an ATF-2 targeting RNAi vector.
- Demonstrated inhibition of HepG2 cell growth and induction of apoptosis via ATF-2 silencing.
- Potential implications for developing new therapies for liver cancer by targeting ATF-2.
