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High molecular weight forms of basic fibroblast growth factor recognized by a new anti-bFGF antibody
C Grothe1, K Zachmann, K Unsicker
1Department of Anatomy and Cell Biology, Philipps-University of Marburg, FRG.
FEBS Letters
|January 15, 1990
Abstract:
An antibody against basic fibroblasts growth factor (bFGF) was raised using purified bovine pituitary bFGF. Western blot analysis revealed immunoreactive bands at 18, 24, 30-33 and 46 kDa in immunoaffinity purified extracts of pituitary and adrenal gland using this antibody. A similar staining pattern was obtained with ovary extracts with the exception of the missing 18 kDa band. A second anti-bFGF antibody raised against a synthetic peptide comprising the 24 N-terminal amino acids of bFGF reacted with the 18 kDa and the 46 kDa band of immunoaffinity purified ovary and adrenal gland extracts.