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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Updated: May 18, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

Aptamer-based molecular imaging.

Tianjiao Wang¹, Judhajeet Ray

¹Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA. tjwang@iastate.edu

|September 18, 2012

Summary

This summary is machine-generated.

Aptamer-based biosensors offer precise molecular imaging for biological research and medicine. These nucleic acid probes enable visualization and quantification of targets in cells and living organisms.

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Area of Science:

Biomedical Engineering
Molecular Biology
Biochemistry

Background:

Molecular imaging is crucial for understanding biological processes and disease.
Aptamers are short nucleic acid sequences with high specificity for molecular targets.
Aptamers are ideal for developing probes for molecular imaging applications.

Purpose of the Study:

To review aptamer-based biosensor development for molecular imaging.
To summarize probe design and imaging modalities.
To discuss applications in imaging small molecules, nucleic acids, and proteins.

Main Methods:

In vitro selection of aptamers for target binding.
Design of aptamer-based probes for imaging.
Application of various imaging modalities.
Inclusion of cellular and animal study data.

Main Results:

Aptamers demonstrate high affinity and specificity for diverse molecular targets.
Aptamer probes have been successfully applied in various molecular imaging techniques.
Applications span imaging of small molecules, nucleic acids, and proteins.

Conclusions:

Aptamer-based molecular imaging is a rapidly advancing field.
These biosensors show significant potential in basic biology and translational medicine.
Future perspectives include further refinement and broader clinical applications.