Related Experiment Video
Updated: May 18, 2026
09:07
Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation
Published on: March 4, 2014
Annexin a6 modulates chick cranial neural crest cell emigration
Chyong-Yi Wu1, Lisa A Taneyhill
1Department of Animal and Avian Sciences, University of Maryland, College Park, Maryland, United States of America.
Plos One
|September 18, 2012
Summary
Annexin A6 regulates the emigration of vertebrate neural crest cells. Modulating Annexin A6 levels affects neural crest cell migration, crucial for development.
Area of Science:
- Developmental Biology
- Cell Biology
- Genetics
Background:
- The vertebrate neural crest is a migratory cell population essential for development.
- Understanding gene regulatory networks governing neural crest cell behavior is critical.
Purpose of the Study:
- To identify key regulators of chick midbrain neural crest cell emigration.
- To investigate the role of Annexin A6 in neural crest cell development.
Main Methods:
- Annexin A6 expression analysis in chick midbrain.
- Depletion and overexpression of Annexin A6 in neural crest cells.
- Assessment of cell migration, proliferation, and epithelial-to-mesenchymal transition (EMT) markers.
Main Results:
- Annexin A6 is expressed in a relevant spatio-temporal pattern.
- Annexin A6 depletion reduced migratory neural crest cell domain; overexpression expanded it.
- Phenotypic changes were linked to premigratory cell population size and EMT markers, not cell death or proliferation.
Conclusions:
- Annexin A6 is a crucial regulator of cranial migratory neural crest cell formation.
- Annexin A6 modulates the epithelial-to-mesenchymal transition and cell migration during vertebrate development.
