Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Methods of Classification and Identification01:28

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploiting Real-Time Genomic Surveillance Data To Assess 4CMenB Meningococcal Vaccine Performance in Scotland, 2015 to 2022.

mBioยท2023
Same author

Parallel Sequencing Reveals Campylobacter spp. in Commercial Meat Chickens Less than 8 Days Old.

Applied and environmental microbiologyยท2021
Same author

Estimated strain coverage of serogroup B meningococcal vaccines: A retrospective study for disease and carrier strains in Greece (2010-2017).

Vaccineยท2021
Same author

Genetic Diversity of Campylobacter jejuni and Campylobacter coli Isolates from Conventional Broiler Flocks and the Impacts of Sampling Strategy and Laboratory Method.

Applied and environmental microbiologyยท2016
Same author

Identifying the seasonal origins of human campylobacteriosis.

Epidemiology and infectionยท2012
Same author

Molecular epidemiology of human Campylobacter jejuni shows association between seasonal and international patterns of disease.

Epidemiology and infectionยท2012
Same journal

Investigating the mobility and host range of mobile genetic elements harbouring antimicrobial resistance genes in enterococci.

Microbiology (Reading, England)ยท2026
Same journal

Commonly prescribed medicines antagonise anti-MRSA antibiotics and select for resistance.

Microbiology (Reading, England)ยท2026
Same journal

Microbial Primer: The T6SS, a deadly bacterial harpoon.

Microbiology (Reading, England)ยท2026
Same journal

Disruption of efflux activity reduces biofilm formation through multiple pathways.

Microbiology (Reading, England)ยท2026
Same journal

Copper-driven mutualism of <i>Candida albicans</i> and <i>Staphylococcus aureus</i> interkingdom biofilms.

Microbiology (Reading, England)ยท2026
Same journal

<i>Entamoeba histolytica</i> spliceosome structural components.

Microbiology (Reading, England)ยท2026
See all related articles

Related Experiment Video

Updated: May 18, 2026

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)
09:43

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)

Published on: October 30, 2016

Campylobacter sequence typing databases: applications and future prospects.

F M Colles1, M C J Maiden1

  • 1Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK.

Microbiology (Reading, England)
|September 19, 2012
PubMed
Summary
This summary is machine-generated.

Human campylobacteriosis, a common cause of gastroenteritis, can be tracked using genetic typing. Multilocus sequence typing (MLST) and antigen gene sequence typing (AGST) identify sources like chicken, aiding intervention strategies.

More Related Videos

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
08:03

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

Related Experiment Videos

Last Updated: May 18, 2026

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)
09:43

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)

Published on: October 30, 2016

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
08:03

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

Area of Science:

  • Microbiology
  • Epidemiology
  • Genetics

Background:

  • Human campylobacteriosis, caused by Campylobacter jejuni and C. coli, is a significant global health concern.
  • Effective interventions are crucial for reducing the disease burden worldwide.

Purpose of the Study:

  • To detail how nucleotide sequence-based typing methods aid in characterizing Campylobacter isolates.
  • To explain the application of these methods in understanding Campylobacter epidemiology and guiding public health interventions.

Main Methods:

  • Utilized multilocus sequence typing (MLST) and antigen gene sequence typing (AGST) for Campylobacter isolate characterization.
  • Analyzed large collections of isolates from diverse sources to understand population structure and epidemiology.
  • Leveraged curated genotype databases (pubMLST.org/campylobacter) for data analysis.

Main Results:

  • Identified clonal complexes within C. jejuni and C. coli populations, linked to specific phenotypes and host predispositions.
  • Determined that retail meat, particularly chicken, is a primary source of human campylobacteriosis in many regions.
  • Demonstrated the utility of sequence-based typing in attributing human isolates to potential sources and informing intervention strategies.

Conclusions:

  • Sequence-based typing provides essential tools for Campylobacter epidemiology, source attribution, and intervention monitoring.
  • Established genotype databases are compatible with emerging whole-genome sequencing (WGS) approaches.
  • The hierarchical structure of MLST and AGST is vital for future WGS data exploitation.