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Updated: May 18, 2026

Genotyping of Plant and Animal Samples without Prior DNA Purification
Published on: September 24, 2012
Automated DNA extraction for large numbers of plant samples
Nataša Mehle1, Petra Nikolić, Matevž Rupar
1National Institute of Biology, Ljubljana, Slovenia. natasa.mehle@nib.si
Abstract:
The method described here is a rapid, total DNA extraction procedure applicable to a large number of plant samples requiring pathogen detection. The procedure combines a simple and quick homogenization step of crude extracts with DNA extraction based upon the binding of DNA to magnetic beads. DNA is purified in an automated process in which the magnetic beads are transferred through a series of washing buffers. The eluted DNA is suitable for efficient amplification in PCR reactions.
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