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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: May 18, 2026

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
14:15

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

Published on: February 23, 2018

Real-time PCR for universal phytoplasma detection and quantification.

Nynne Meyn Christensen1, Henriette Nyskjold, Mogens Nicolaisen

  • 1Center for Advanced Bioimaging, University of Copenhagen, Copenhagen, Denmark.

Methods in Molecular Biology (Clifton, N.J.)
|September 19, 2012
PubMed
Summary
This summary is machine-generated.

Real-time PCR offers efficient phytoplasma detection and quantification, surpassing nested PCR in sensitivity and ease of use. A universal real-time PCR method is crucial for large-scale screening programs.

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Last Updated: May 18, 2026

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
14:15

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

Published on: February 23, 2018

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08:37

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Published on: March 30, 2015

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07:25

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Published on: June 25, 2020

Area of Science:

  • Plant pathology
  • Molecular biology
  • Microbial diagnostics

Background:

  • Phytoplasmas are plant pathogens causing significant agricultural losses.
  • Accurate detection and quantification are essential for disease management.
  • Current methods like nested PCR are labor-intensive and prone to contamination.

Purpose of the Study:

  • To highlight the advantages of real-time PCR for phytoplasma detection.
  • To emphasize the need for a universal real-time PCR assay.
  • To advocate for the adoption of real-time PCR in screening programs.

Main Methods:

  • Real-time PCR (polymerase chain reaction)
  • Comparison with nested PCR techniques
  • Evaluation of sensitivity and work intensity

Main Results:

  • Real-time PCR is the most efficient method for phytoplasma detection and quantification.
  • Real-time PCR demonstrates higher sensitivity and is less labor-intensive than nested PCR.
  • Reduced susceptibility to contamination compared to traditional PCR methods.

Conclusions:

  • Real-time PCR provides a superior alternative for phytoplasma analysis.
  • A universal real-time PCR assay would greatly benefit phytoplasma screening programs.
  • This method enhances diagnostic efficiency in high-throughput settings.