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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

Plasmids

Plasmids

Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...

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Related Experiment Video

Updated: May 18, 2026

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

Published on: January 20, 2017

Phytoplasma plasmid DNA extraction.

Mark T Andersen¹, Lia W Liefting

¹Plant & Food Research, Auckland, New Zealand. mandersen@hortresearch.co.nz

Methods in Molecular Biology (Clifton, N.J.)

|September 19, 2012

Summary

This summary is machine-generated.

This study details two specific methods for isolating phytoplasma plasmid DNA. These techniques improve upon general DNA extraction methods for better detection in plants and insects.

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Last Updated: May 18, 2026

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

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Published on: January 20, 2017

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Published on: March 26, 2011

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

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Published on: July 27, 2018

Area of Science:

Plant pathology
Microbial genetics
Molecular biology

Background:

Phytoplasma plasmids are typically detected using general phytoplasma DNA extraction methods.
Existing methods often involve purification from high-titer tissues like plant phloem.
Techniques like CsCl-bisbenzimide gradients exploit phytoplasma DNA's low G+C content.

Purpose of the Study:

To describe two detailed methods specifically for isolating phytoplasma plasmid DNA.
To provide improved techniques for phytoplasma research.

Main Methods:

Detailed description of two novel plasmid DNA isolation protocols.
Adaptation of existing phytoplasma DNA purification strategies.

Main Results:

The presented methods are specifically designed for phytoplasma plasmid isolation.
These protocols offer enhanced specificity compared to general DNA extraction.

Conclusions:

The described methods provide targeted approaches for phytoplasma plasmid DNA isolation.
These techniques are valuable for researchers studying phytoplasma genetics and pathogenicity.