Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Determination01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Internal connectivity of the mouse mesocortical ring and functional implications.

Brain structure & function·2026
Same author

Morphogenetic Patterning During Regional and Cell Type Specification in the Embryonic Basal Ganglia.

bioRxiv : the preprint server for biology·2026
Same author

Field Homology in the Brain of Vertebrates.

Biology·2026
Same author

Transcriptomics reveals pallial and subpallial subdivisions of the mouse medial amygdala.

Brain structure & function·2026
Same author

Ramón y Cajal's 'lenticular tract' represents infrasubthalamic pyramidal collaterals targeting mesodiencephalic centers: an obscure misunderstood aspect of the motor pathway clarified by Allen Mouse Brain Connectivity data.

Frontiers in neuroanatomy·2025
Same author

Transcriptomic Analysis Corroborates the New Radial Model of the Mouse Pallial Amygdala.

Biomolecules·2025

Related Experiment Video

Updated: May 18, 2026

In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development
10:09

In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development

Published on: June 16, 2022

Fgf10 expression patterns in the developing chick inner ear.

Luis Óscar Sánchez-Guardado1, Luis Puelles, Matías Hidalgo-Sánchez

  • 1Department of Cell Biology, School of Science, University of Extremadura, Badajoz E06071, Spain.

The Journal of Comparative Neurology
|September 19, 2012
PubMed
Summary

The gene Fgf10 is crucial for developing inner ear sensory organs in birds. Its expression pattern reveals how a single domain subdivides to form multiple sensory patches during development.

More Related Videos

Selective Tracing of Auditory Fibers in the Avian Embryonic Vestibulocochlear Nerve
11:27

Selective Tracing of Auditory Fibers in the Avian Embryonic Vestibulocochlear Nerve

Published on: March 18, 2013

Gene Transfer into the Chicken Auditory Organ by In Ovo Micro-electroporation
06:45

Gene Transfer into the Chicken Auditory Organ by In Ovo Micro-electroporation

Published on: April 17, 2016

Related Experiment Videos

Last Updated: May 18, 2026

In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development
10:09

In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development

Published on: June 16, 2022

Selective Tracing of Auditory Fibers in the Avian Embryonic Vestibulocochlear Nerve
11:27

Selective Tracing of Auditory Fibers in the Avian Embryonic Vestibulocochlear Nerve

Published on: March 18, 2013

Gene Transfer into the Chicken Auditory Organ by In Ovo Micro-electroporation
06:45

Gene Transfer into the Chicken Auditory Organ by In Ovo Micro-electroporation

Published on: April 17, 2016

Area of Science:

  • Developmental biology
  • Neuroscience
  • Evolutionary biology

Background:

  • The inner ear develops from the otic placode, forming sensory structures for hearing and balance.
  • Evolutionary theories suggest sensory patches arose from an early, widespread sensory domain.
  • Understanding the genetic mechanisms of inner ear development is key to evolutionary insights.

Purpose of the Study:

  • To analyze the spatial and temporal expression of Fgf10 during inner ear development.
  • To compare Fgf10 expression with markers of otic patterning and hair cell differentiation.
  • To elucidate the developmental basis for the formation of distinct sensory organs in the inner ear.

Main Methods:

  • Detailed analysis of Fgf10 gene expression patterns in chick embryos.
  • Comparison of Fgf10 expression with established markers for otic patterning (e.g., Serrate).
  • Observation of hair cell differentiation markers to correlate with Fgf10 activity.

Main Results:

  • Fgf10 expression initially marks a ventromedial band in the otocyst, a sensory-competent domain.
  • This Fgf10-expressing area subdivides to form six of the eight avian inner ear sensory organs.
  • The lateral crista and macula neglecta initially lack Fgf10 but activate it post-specification.

Conclusions:

  • Fgf10 plays a critical role in specifying distinct sensory epithelia within the developing inner ear.
  • The subdivision of an Fgf10-expressing domain provides a mechanism for generating multiple sensory organs.
  • This study establishes a developmental timetable for sensory specification in the chick inner ear and offers evolutionary insights.