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Updated: May 18, 2026
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Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells In Vitro and In Vivo
Published on: February 5, 2021
DEC-205 is a cell surface receptor for CpG oligonucleotides
Mireille H Lahoud1, Fatma Ahmet, Jian-Guo Zhang
1Centre for Immunology, Burnet Institute, Melbourne, VIC 3004, Australia.
Summary
DEC-205 acts as a receptor for CpG oligonucleotides (ODN), enhancing their uptake and cellular delivery. This discovery impacts vaccine adjuvant development by revealing a key mechanism for CpG ODN function.
Area of Science:
- Immunology
- Cell Biology
- Vaccinology
Background:
- Synthetic CpG oligonucleotides (ODN) are potent immunostimulants used in vaccine trials.
- The cellular uptake and delivery mechanisms of CpG ODN to Toll-like receptor 9 (TLR9) remain unclear.
Purpose of the Study:
- To identify the receptor responsible for CpG ODN capture and delivery.
- To elucidate the role of this receptor in immune responses mediated by CpG ODN.
Main Methods:
- Utilized mice deficient in DEC-205 to assess immune responses.
- Investigated direct binding and uptake of CpG ODN by DEC-205.
- Compared CpG ODN binding by murine and human DEC-205.
Main Results:
- DEC-205 was identified as a direct receptor for class B CpG ODN, enhancing their uptake.
- Mice lacking DEC-205 showed impaired dendritic cell and B-cell maturation.
- These mice exhibited reduced cytokine production (e.g., IL-12) and suboptimal T-cell responses when CpG ODN were used as adjuvants.
- Human DEC-205 preferentially binds specific clinical-grade CpG ODN.
Conclusions:
- DEC-205 is a crucial receptor for class B CpG ODN.
- DEC-205 plays a significant role in mediating CpG ODN-induced immune responses.
- This finding offers new insights into vaccine adjuvant design and delivery.
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