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Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil

Teresinha Teixeira de Sousa1, Menira Souza, Fabíola Souza Fiaccadori

  • 1Laboratório de Virologia Humana, Instituto de Patologia Tropical e Saúde Pública, Goiânia, GO, Brasil.

Memorias Do Instituto Oswaldo Cruz
|September 20, 2012
PubMed

Insights

Human bocavirus (HBoV) 1 and 3 were detected in 5.8% of children with acute gastroenteritis in Brazil. Co-infections with other viruses were common, highlighting HBoV

Area of Science:

  • Virology
  • Pediatrics
  • Epidemiology

Background:

  • Human bocavirus (HBoV) is an emerging pathogen implicated in respiratory and gastrointestinal infections in children.
  • Limited data exists on HBoV prevalence in South America, particularly in Brazil.
  • Understanding HBoV epidemiology is crucial for diagnosing and managing pediatric gastroenteritis.

Purpose of the Study:

  • To determine the prevalence of human bocavirus (HBoV) types 1 and 3 in children with acute gastroenteritis in Brazil's Central-West Region.
  • To characterize the genetic diversity and phylogenetic relationships of detected HBoV strains.
  • To investigate co-infections with other common enteric viruses.

Main Methods:

  • Retrospective analysis of 762 fecal samples from children with acute gastroenteritis (1994-2004).
  • Polymerase chain reaction (PCR) using primers for the NS1 gene of HBoV-1 and HBoV-3.
  • Genomic sequencing and phylogenetic analysis of positive samples.

Main Results:

  • HBoV-1 or HBoV-3 DNA was detected in 5.8% (44/762) of fecal samples.
  • Co-infection was observed in 31.8% (14/44) of HBoV-positive samples, with Rotavirus A and Aichi virus being common.
  • Phylogenetic analysis revealed high sequence homology of HBoV-1 strains with those found globally, including Brazil.

Conclusions:

  • HBoV-1 and HBoV-3 are present in children with acute gastroenteritis in Brazil's Central-West Region.
  • Co-infections with other enteric viruses are frequent, suggesting complex etiologies for pediatric gastroenteritis.
  • Further research is needed to elucidate the etiological role of HBoVs in gastroenteritis.

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