High throughput sequencing approaches to mutation discovery in the mouse.

Area of Science:

• Genetics and Genomics
• Molecular Biology
• Developmental Biology

Background:

• Phenotype-driven research in mice is crucial for understanding gene function and biological complexity.
• Traditional mutation discovery methods are effective but labor-intensive and time-consuming.
• High-throughput sequencing (HTS) technologies have transformed forward genetics in mice, enabling faster mutation discovery.

Purpose of the Study:

• To review current high-throughput sequencing applications and resources for mutation discovery in mice.
• To provide an overview of practical considerations for implementing HTS and analyzing the resulting data.

Main Methods:

• Review of current literature on high-throughput sequencing technologies applied to mouse forward genetics.
• Analysis of strategies for mutation discovery and data analysis in the context of HTS.

Main Results:

• HTS technologies significantly accelerate the process of identifying mutations in mice.
• The choice of sequencing approach and data analysis strategies are critical for successful HTS application.
• Various resources and practical considerations are available to guide HTS implementation.

Conclusions:

• HTS is a powerful tool for accelerating gene discovery and unraveling biological mechanisms in mice.
• Effective implementation of HTS requires careful consideration of sequencing methods and data analysis pipelines.
• This review provides a guide for researchers utilizing HTS in mouse forward genetics studies.