Related Experiment Video
Updated: May 18, 2026

Study of Endoplasmic Reticulum and Mitochondria Interactions by In Situ Proximity Ligation Assay in Fixed Cells
Published on: December 10, 2016
Endoplasmic reticulum-mitochondria contacts: function of the junction
Ashley A Rowland1, Gia K Voeltz
1Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, 80309, USA.
Abstract:
The most well-characterized organelle contact sites are those between the endoplasmic reticulum (ER) and mitochondria. Increased understanding is being gained of how ER-mitochondria contact sites are organized and which factors converge at this interface, some of which may provide a tethering function. The role of the ER-mitochondria junction in coordinating the functions of these two organelles is also becoming clearer, and it has been shown to be involved in the regulation of lipid synthesis, Ca(2+) signalling and the control of mitochondrial biogenesis and intracellular trafficking.
Related Concept Videos
The Inner Mitochondrial Membrane
Mitochondrial Membranes
Mitochondrial Membranes
Translocation of Proteins into the Mitochondria
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Endoplasmic Reticulum
Contact-dependent Signaling
Gap Junctions
In animal cells, gap junctions are formed...

