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Updated: May 18, 2026
Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins
Published on: September 1, 2013
Intravital microscopy: a practical guide on imaging intracellular structures in live animals
Andrius Masedunskas1, Oleg Milberg2, Natalie Porat-Shliom3
1Intracellular Membrane Trafficking Unit; Oral and Pharyngeal Cancer Branch; National Institute of Dental and Craniofacial Research; National Institutes of Health; Bethesda, MD USA; Department of Biology; University of North Carolina at Chapel Hill; Chapel Hill, NC USA.
Intravital microscopy allows researchers to visualize cellular processes in live animals. This review offers practical guidance for new users on techniques for subcellular imaging in various organs.
Area of Science:
- Life Sciences
- Cell Biology
- Microscopy
Background:
- Intravital microscopy enables in vivo imaging of biological processes.
- Advancements in genetic tools and imaging capabilities facilitate subcellular resolution in live animals.
- Cell biology research benefits from in vivo imaging techniques.
Purpose of the Study:
- To provide an overview of intravital microscopy for researchers new to the field.
- To highlight the potential and challenges of intravital microscopy.
- To offer practical guidance on performing live animal imaging.
Main Methods:
- Discusses selection of imaging platforms and modalities.
- Covers surgical and stabilization techniques for live animal imaging.
- Details anesthesia and temperature control strategies.
Main Results:
- Demonstrates the feasibility of subcellular imaging in multiple organs.
- Provides examples of imaging organelles and the actin cytoskeleton in vivo.
- Shares practical know-how from first-hand experience.
Conclusions:
- Intravital microscopy is a powerful tool for cell biology research.
- Practical considerations are crucial for successful live animal imaging.
- This review equips researchers with essential knowledge for applying intravital microscopy.
