Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

In Vitro Fertilization

In Vitro Fertilization

In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Repositioning Non-Ablative Fractional Lasers - Molecular and Histological Insights from a scoping review.

Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·2026

Same author

[Fundamentals of local anesthesia].

Dermatologie (Heidelberg, Germany)·2026

Same author

[Tumescent and nerve block anesthesia in dermatosurgery].

Dermatologie (Heidelberg, Germany)·2026

Same author

Retrospective case series of two-stage folded paramedian forehead flap nasal reconstruction without cartilage grafts.

Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·2026

Same author

Diagnostic Accuracy of Ex Vivo Confocal Laser Scanning Microscopy for Routine Detection of Cutaneous Squamous Cell Carcinoma and Actinic Keratoses.

Cancers·2026

Same author

Frequency of squamous cell carcinomas in vermilionectomy specimens after confirmed actinic cheilitis.

Journal of the European Academy of Dermatology and Venereology : JEADV·2026

Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 18, 2026

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

Sperm selection for ICSI using annexin V.

Sonja Grunewald¹, Uwe Paasch

¹EAA Training Center, Klinik und Poliklinik für Dermatologie, Venerologie und Allergologie, Universitätsklinikum Leipzig AöR, Leipzig, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|September 21, 2012

Summary

This summary is machine-generated.

Annexin-V magnetic-activated cell sorting (MACS) improves sperm selection for assisted reproduction by identifying damaged sperm. Combining Annexin-V MACS with density gradient centrifugation boosts clinical pregnancy rates in intracytoplasmic sperm injection (ICSI) cycles.

More Related Videos

Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

Related Experiment Videos

Last Updated: May 18, 2026

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

Area of Science:

Reproductive medicine
Cell biology
Assisted reproductive technology

Background:

Sperm quality is crucial for successful assisted reproduction.
Apoptosis and membrane damage can negatively impact sperm function.
Current sperm selection methods may not fully exclude compromised spermatozoa.

Purpose of the Study:

To detail the methodology of Annexin-V magnetic-activated cell sorting (MACS) for sperm selection.
To highlight Annexin-V MACS as a tool to optimize sperm selection in assisted reproduction.
To present Annexin-V MACS as a method to identify sperm with activated apoptosis or membrane damage.

Main Methods:

Annexin-V magnetic-activated cell sorting (MACS) utilizes superparamagnetic Annexin-microbeads.
These beads bind to externalized phosphatidylserine on sperm with activated apoptosis or membrane damage.
The technique is often combined with density gradient centrifugation for enhanced sperm selection.

Main Results:

Annexin-V MACS effectively identifies sperm exhibiting signs of apoptosis or membrane damage.
The combination of Annexin-V MACS and density gradient centrifugation has been shown to improve outcomes.
Enhanced clinical pregnancy rates were observed in intracytoplasmic sperm injection (ICSI) cycles using this combined approach.

Conclusions:

Annexin-V MACS is a valuable technique for optimizing sperm selection in assisted reproduction.
This method aids in the exclusion of sperm with compromised membrane integrity.
The integration of Annexin-V MACS with established methods like density gradient centrifugation offers significant clinical benefits, including higher pregnancy rates.