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Updated: May 18, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
Published on: January 7, 2017
Rapid and direct detection of attomole adenosine triphosphate (ATP) by MALDI-MS using rutile titania chips
Muthu Manikandan1, Nazim Hasan, Hui-Fen Wu
1Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan.
Abstract:
We report the rutile titania-based capture of ATP and its application as a MALDI-MS target plate. This chip, when immersed in solutions containing different concentrations of ATP, can capture ATP and lead to its successful detection in MALDI-MS. We have optimized the ideal surface, showing an increased capture efficacy of the 900 °C (rutile) titania surfaces. We demonstrate the use of this chip as a target plate for direct analysis of the attached ATP using MALDI-MS, down to attomolar concentrations. This chip has a promising future for the detection of ATP in environmental samples, which may eventually be used as a pollution indicator in particular environments.
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