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[Quantitative determination of sibricose A5 and sibricose A6 in Polygalae radix]
Changqing Sun1, Xiaomin Peng, Nailiang Zhu
1School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. sunchangqingscq@126.com
Objective:
To establish the method for quantitative determination of sibricose A5 and sibricose A6 in Polygalae Radix by HPLC.
Method:
The ultrasonic extracting method was applied in sample pre-treatment. The HPLC procedure was performed on the chromatographic column of Agela Promosil C18 (4.6 mm x 250 mm, 5 microm), the mobile phase was acetonitrile-0.1% phosphoric acid water solution (10:90). The detection wavelength was 330 nm and flow velocity was 1 mL x min(-1). The column temperature was 30 degrees C.
Result:
The method has good linearity in the ranges of 0.0087-0.0694 g x L(-1) (r = 0.9993) for sibricose A5, 0.0090-0.0723 g x L(-1) (r=0.9991) for sibricose A6. The average recoveries of sibricose A5 and sibricose A6 were 101.7%, 97.87%, with the RSD of 1.7%, 1.6%, respectively.
Conclusion:
The method was simple, quick accurate and reliable. It is appropriate for the quantitative determination of sibricose A5 and sibricose A6 in Polygalae Radix.
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