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Related Concept Videos

DNA Helicases00:55

DNA Helicases

DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Complex Assembly02:41

Protein Complex Assembly

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Many viruses self-assemble into a fully functional unit using the infected host cell to...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:23

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The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:19

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The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
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There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...

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Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
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Ski2-like RNA helicase structures: common themes and complex assemblies.

Sean J Johnson1, Ryan N Jackson

  • 1Department of Chemistry & Biochemistry, Utah State University, Logan, UT, USA. sean.johnson@usu.edu

RNA Biology
|September 22, 2012
PubMed
Summary
This summary is machine-generated.

New structural insights reveal Ski2-like RNA helicases share a conserved ring-like architecture. These intricate proteins, including Mtr4, Ski2, and Brr2, integrate into larger complexes crucial for RNA processing and degradation.

Keywords:
Brr2Mtr4RNA helicaseRNA processingSki2structure

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Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biochemistry

Background:

  • Ski2-like RNA helicases are large, multidomain proteins essential for RNA metabolism.
  • Previous structural data for these helicases were limited.

Purpose of the Study:

  • To review recent structural findings of Mtr4, Ski2, and Brr2.
  • To elucidate the conserved architecture and functional roles of Ski2-like RNA helicases.

Main Methods:

  • Structural analysis of Mtr4, Ski2, and Brr2.
  • Comparative structural studies of apo- and RNA-bound Mtr4.

Main Results:

  • A conserved ring-like architecture, including RecA, winged helix, and ratchet domains, was revealed.
  • The winged helix domain acts as a molecular hub coordinating RNA interactions.
  • Unique accessory domains contribute to family-specific functions.

Conclusions:

  • Ski2-like RNA helicases possess a conserved core structure with diverse accessory domains.
  • These helicases are integral components of larger protein assemblies, such as the exosome.
  • Structural insights facilitate understanding of RNA processing and degradation complexes.