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Updated: May 18, 2026

Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain

Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain

Published on: December 22, 2014

Tracing synaptic connectivity onto embryonic stem cell-derived neurons.

Isabella Garcia¹, Longwen Huang, Kevin Ung

¹Program in Developmental Biology, Baylor College of Medicine, Houston, Texas 77030, USA.

Stem Cells (Dayton, Ohio)

|September 22, 2012

Summary

This summary is machine-generated.

Researchers developed genetically engineered mouse stem cells for advanced neural circuit tracing. These cells enable precise mapping of synaptic connections, aiding neuroscience research and cell replacement therapy development.

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Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain

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Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells

Published on: August 16, 2018

Area of Science:

Neuroscience
Stem Cell Biology
Molecular Biology

Background:

Transsynaptic circuit tracing with rabies virus (RV) is crucial for understanding neural connections.
Embryonic stem cell (ESC)-derived neurons are vital for cell replacement therapies and studying neuronal properties.
Existing methods lack integrated tools for tracing synaptic input onto specific neuronal populations.

Purpose of the Study:

To generate a novel mouse ESC line engineered for rabies virus-mediated transsynaptic circuit tracing.
To enable precise identification of presynaptic inputs onto ESC-derived neurons.
To facilitate research into synapse and neural circuit formation.

Main Methods:

Gene targeting to introduce tdTomato, Rabies-G glycoprotein, and avian TVA receptor into the ROSA26 locus of mouse ESCs.
Differentiation of engineered mESCs into functional neurons.
In vitro and in vivo experiments to demonstrate transsynaptic tracing capabilities and synaptic integration.

Main Results:

Successful generation of a novel mESC line with genetic elements for RV-based tracing.
High-efficiency differentiation into functional neurons capable of forming synaptic connections.
Demonstrated ability of ESC-derived neurons to serve as source cells for presynaptic tracing in vitro and in vivo.

Conclusions:

The engineered mESC line provides a powerful tool for studying neural circuits and synapse formation.
This technology facilitates unambiguous identification of presynaptic inputs onto specific neuronal populations.
The developed system holds significant potential for advancing cell replacement therapies and fundamental neuroscience research.