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Related Experiment Video

Updated: May 18, 2026

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
10:45

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Published on: June 14, 2013

Transcriptome-wide analysis of exosome targets.

Claudia Schneider1, Grzegorz Kudla, Wiebke Wlotzka

  • 1Wellcome Trust Centre for Cell Biology, The University of Edinburgh, Edinburgh UK. claudia.schneider@ncl.ac.uk

Molecular Cell
|September 25, 2012
PubMed
Summary

The yeast exosome degrades various RNAs, including non-coding RNAs and pre-mRNAs. This study reveals RNA crosslinking (CRAC) identifies exosome targets and degradation pathways.

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Last Updated: May 18, 2026

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
10:45

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Published on: June 14, 2013

Isolation and Characterization of RNA-Containing Exosomes
09:43

Isolation and Characterization of RNA-Containing Exosomes

Published on: January 9, 2012

An Innovative Method for Exosome Quantification and Size Measurement
11:38

An Innovative Method for Exosome Quantification and Size Measurement

Published on: January 17, 2015

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • RNA Metabolism

Background:

  • The exosome complex is crucial for RNA processing and surveillance.
  • In vivo mechanisms of exosome substrate acquisition remain largely unknown.

Purpose of the Study:

  • To investigate the in vivo target range of the yeast exosome.
  • To elucidate the substrate acquisition and degradation mechanisms employed by the exosome.

Main Methods:

  • In vivo RNA crosslinking followed by analysis of immunoprecipitated sequences (CRAC).
  • CRAC was applied to key exosome components: Rrp44, Rrp6, Rrp41, Csl4, and Trf4.
  • Split-CRAC using cleavable proteins was employed to differentiate nuclease activities.

Main Results:

  • CRAC identified CUT and SUT non-coding RNAs, snoRNAs, pre-tRNAs, Pol III transcripts, and unspliced pre-mRNAs as exosome targets.
  • Rrp44 and Rrp6 were implicated in the degradation of these diverse RNA substrates.
  • Rrp44 exhibits both endonuclease and exonuclease activities, potentially releasing stalled substrates.
  • Rrp6 preferentially targets structured RNAs, suggesting alternative substrate processing pathways.

Conclusions:

  • The yeast exosome targets a broad spectrum of RNA molecules for degradation.
  • Exosome-mediated RNA degradation involves coordinated nuclease activities and multiple substrate pathways.
  • Understanding these pathways is critical for comprehending RNA homeostasis and quality control in vivo.