Effect of tricalcium silicate (Ca(3)SiO(5)) bioactive material on reducing enamel demineralization: an in vitro...

The aim of this study was to investigate the effect of Ca(3)SiO(5) on reducing enamel demineralization under pH-cycling conditions.

Forty bovine enamel samples were treated under four conditions: group 1, double distilled water (negative control); group 2, 1000 ppm F (as NaF, positive control); group 3, Ca(3)SiO(5) slurry; and group 4, Ca(3)SiO(5)-F slurry (Ca(3)SiO(5) with 1000 ppm F aq.). All the specimens were treated with treatment materials 4 times each day. Samples in groups 1 and 2 were soaked in test solutions for 2 min and samples in groups 3 and 4 were painted in treatment slurry for 2 min. At times between treatments, they were immersed in citric acid solution 3 times a day and 15 s each time. All the procedures were repeated for 7 days. Knoop microhardness, scanning electron microscopy (SEM), X-ray diffraction (XRD) and atom force microscopy (AFM) were used to examine samples.

After treatment for 7 days, enamels in all the groups were significantly softened. The extents of microhardness reduction were 52.3%, 28.5%, 28.5% and 20.2% for groups 1, 2, 3 and 4, respectively. Samples in the negative control group showed a typical acid etched pattern while enamels in other groups were relatively compact. There was no significant difference between samples treated with Ca(3)SiO(5) and F. The combination of Ca(3)SiO(5) with F showed the best effect on reducing enamel demineralization.

Ca(3)SiO(5) is an effective material against enamel demineralization alone but in combination with F a better anti-demineralization effect may be obtained.