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Updated: May 18, 2026

12:11
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
Published on: February 27, 2020
Direct immobilization of oxyamine-modified proteins from cell lysates
Long Yi1, Yong-Xiang Chen, Po-Chiao Lin
1Department of Chemical Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, D-44227 Dortmund, Germany.
Abstract:
Oxyamine-modified proteins can be efficiently and selectively immobilized on ketone-coated glass slides at neutral pH in short reaction times by direct treatment and spotting of protein expression lysates without prior purification.

