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Updated: May 18, 2026

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

Published on: June 27, 2022

Coumarin-based fluorescent probes for H2S detection.

Wenhua Li¹, Wei Sun, Xiaoqiang Yu

¹Department of Medicinal Chemistry, Key Laboratory of Chemical Biology of Natural Products (MOE), School of Pharmacy, Shandong University, Jinan, Shandong 250012, China.

Journal of Fluorescence

|September 25, 2012

Summary

This summary is machine-generated.

Scientists developed new coumarin-based fluorescent probes to detect hydrogen sulfide (H2S). Probe 1b shows high selectivity and sensitivity for H2S in vitro and in living cells, enabling better physiological monitoring.

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Area of Science:

Chemical Biology
Analytical Chemistry
Biochemistry

Background:

Hydrogen sulfide (H2S) is recognized for its toxicity and distinct odor.
Emerging evidence highlights H2S's crucial role in physiological processes.
There is a growing need for precise methods to monitor H2S in biological systems.

Purpose of the Study:

To design and synthesize novel coumarin-based fluorescent probes for H2S detection.
To evaluate the selectivity and sensitivity of these probes for H2S.
To demonstrate the application of the probes for identifying H2S in living cells.

Main Methods:

Synthesis of coumarin-based compounds 1a and 1b.
Evaluation of probe response to H2S via fluorescence spectroscopy.
Assessment of probe selectivity against other reactive sulfur species.
In vitro and in vivo imaging experiments in living cells.

Main Results:

Probes 1a and 1b were successfully synthesized and characterized.
Probe 1b exhibited high selectivity and sensitivity towards H2S.
Probe 1b effectively detected exogenous H2S in living cells.
The probes function through the reduction of azide to amine by H2S.

Conclusions:

Developed coumarin-based fluorescent probes offer a promising tool for H2S detection.
Probe 1b demonstrates excellent performance for selective and sensitive H2S sensing.
These probes facilitate the study of H2S in biological contexts, including live cell imaging.