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Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
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Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

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Label-free microscale thermophoresis discriminates sites and affinity of protein-ligand binding

Susanne A I Seidel1, Christoph J Wienken, Sandra Geissler

  • 1Systems Biophysics and Functional Nanosystems, Ludwig-Maximilians-Universität München, Amalienstrasse 54, 80799 Munich, Germany.

Angewandte Chemie (International Ed. in English)
|September 25, 2012
PubMed

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The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium: