Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 18, 2026

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads
07:13

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Published on: June 28, 2024

Single quantum dot based nanosensor for renin assay.

Yi Long1, Ling-fei Zhang, Yan Zhang

  • 1Single-Molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Guangdong 518055, China.

Analytical Chemistry
|September 26, 2012
PubMed
Summary

We developed a novel single quantum dot (QD) nanosensor for sensitive renin activity detection. This high-sensitivity sensor offers a low-cost method for diagnosing renin-related diseases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Construction of a Glutathione-Gated Intelligent Nanoclassifier for Spatioselective Visualization of Dual CircRNAs and Synergistic Photodynamic Therapy.

Angewandte Chemie (International ed. in English)·2026
Same author

State-of-the-Art Biosensors in China.

Biosensors·2026
Same author

Recent advances in graphene-based field-effect transistor sensors for the detection of biomolecules and organic molecules.

Chemical communications (Cambridge, England)·2026
Same author

A bisulfite-free sequence-independent biosensor for genomic 5-hydroxymethylcytosine detection via triple-cascade signal amplification.

Biosensors & bioelectronics·2026
Same author

Construction of Codirectional Charge Transfer Channels in Imine-Linked Covalent Organic Frameworks with Enhanced Electrochemiluminescence for Ultrasensitive Lincomycin Assay.

ACS sensors·2026
Same author

Design and optimization of an HVAC-integrated ozone disinfection system for the purified buffer room of PIVAS.

iScience·2026

Area of Science:

  • Biomedical Engineering
  • Biophysics
  • Biochemistry

Background:

  • Plasma renin activity is crucial for diagnosing renin-related diseases like hypertension and heart failure.
  • Current methods for renin activity evaluation can be complex and require significant sample volumes.

Purpose of the Study:

  • To develop a highly sensitive and cost-effective nanosensor for quantifying renin activity.
  • To enable single-molecule level analysis of renin concentration and enzymatic kinetics.

Main Methods:

  • A single quantum dot (QD) based nanosensor was engineered using streptavidin-coated QDs and biotinylated, Cy5-labeled peptide substrates.
  • Fluorescence resonance energy transfer (FRET) between the QD donor and Cy5 acceptor was utilized.
  • Total internal reflection fluorescence (TIRF) microscopy measured Cy5 counts to assess renin activity.

More Related Videos

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
10:50

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

CRISPR-Cas-mediated Multianalyte Synthetic Urine Biomarker Test for Portable Diagnostics
04:33

CRISPR-Cas-mediated Multianalyte Synthetic Urine Biomarker Test for Portable Diagnostics

Published on: December 8, 2023

Related Experiment Videos

Last Updated: May 18, 2026

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads
07:13

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Published on: June 28, 2024

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
10:50

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

CRISPR-Cas-mediated Multianalyte Synthetic Urine Biomarker Test for Portable Diagnostics
04:33

CRISPR-Cas-mediated Multianalyte Synthetic Urine Biomarker Test for Portable Diagnostics

Published on: December 8, 2023

Main Results:

  • The nanosensor demonstrated high sensitivity with a detection limit of 25 pM for renin activity.
  • The system allowed for quantitative measurement of renin concentration, enzymatic velocity, and Michaelis-Menten kinetic parameters.
  • Renin presence caused substrate cleavage, leading to decreased FRET efficiency and reduced Cy5 counts.

Conclusions:

  • The single-QD nanosensor provides a simple, low-cost, and highly sensitive method for evaluating renin activity.
  • This technology has potential applications in diagnosing renin-related diseases and monitoring other enzymatic biomarkers.