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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...

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mirMachine: A One-Stop Shop for Plant miRNA Annotation
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Published on: May 1, 2021

Setting up an intronic miRNA database.

Ludwig Christian Hinske1, Jens Heyn, Pedro A F Galante

  • 1Department of Anaesthesiology, Clinic of the University of Munich, Munich, Germany. ludwig.hinske@med.uni-muenchen.de

Methods in Molecular Biology (Clifton, N.J.)
|September 26, 2012
PubMed
Summary
This summary is machine-generated.

Intragenic microRNAs (miRNAs) linked to host genes provide unique biological insights. This study details database creation and data integration methods for challenging intronic miRNA genome-wide analysis.

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Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Intragenic microRNAs (miRNAs) are gaining attention due to their linkage with host gene transcription.
  • This association offers unique insights into host gene properties compared to intergenic miRNAs.
  • Genome-wide analysis of intronic miRNA data presents significant challenges, particularly with web-based tools.

Purpose of the Study:

  • To describe the establishment of a database for intronic miRNA analysis.
  • To demonstrate linking publicly available information resources for miRNAs and their host genes.
  • To provide a practical example of an intronic miRNA analysis technique.

Main Methods:

  • Database setup for intronic miRNA and host gene data.
  • Integration of diverse, publicly available biological information resources.
  • Application of a straightforward yet effective analysis technique for intronic miRNA data.

Main Results:

  • A functional database structure for intronic miRNA analysis was successfully established.
  • The linkage of miRNA and host gene data resources was demonstrated.
  • A simple analysis technique was successfully applied, yielding useful insights.

Conclusions:

  • The proposed database structure and integration methods facilitate challenging intronic miRNA genome-wide analysis.
  • The presented techniques can be extended to incorporate additional data types and analysis methods.
  • This approach enhances the utility of intragenic miRNAs as biomarkers and for understanding gene regulation.