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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Related Experiment Video

Updated: May 18, 2026

Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice
12:54

Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice

Published on: May 5, 2014

Transient, inducible, placenta-specific gene expression in mice.

Xiujun Fan1, Matthew Petitt, Matthew Gamboa

  • 1Department of Obstetrics and Gynecology, Stanford Cancer Center, Stanford University, California 94305-5317, USA.

Endocrinology
|September 27, 2012
PubMed
Summary

Researchers developed a new inducible system for placenta-specific gene expression in mice. This allows transient gene control and live monitoring during pregnancy, aiding studies of placental development and disorders.

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Published on: August 15, 2011

Area of Science:

  • Reproductive biology
  • Developmental biology
  • Genetics

Background:

  • Understanding placental function and pregnancy disorders is hindered by a lack of placenta-specific gene manipulation tools.
  • Existing lentivirus methods provide persistent, but not controllable, gene expression, limiting functional analysis of stage-specific genes.

Purpose of the Study:

  • To develop a novel inducible, placenta-specific gene expression system for transient transgene expression and live monitoring in mouse placenta.
  • To enable functional analysis of developmentally and physiologically important placental genes at specific pregnancy stages.

Main Methods:

  • Utilized the highly sensitive third-generation tetracycline-inducible transactivator (Tet-On 3G) system.
  • Created transgenic mice using an integrase-based, site-specific approach for high-level transgene expression.
  • Employed lentivirus-mediated gene delivery into blastocysts for placenta-specific, doxycycline (Dox)-inducible expression of firefly luciferase.

Main Results:

  • Achieved high-level, transient, and placenta-specific transgene expression as early as day 5 of pregnancy.
  • Demonstrated Dox dose-dependent induction and on-off control of gene expression in vivo.
  • Enabled live monitoring of gene expression in the mouse placenta throughout pregnancy using bioluminescence imaging.

Conclusions:

  • The developed system provides reliable, stage-specific induction and monitoring of gene expression in the placenta.
  • This breakthrough facilitates studies on placental development, function, and pregnancy disorders.
  • The site-specific Tet-On 3G mouse model offers broad applicability for gene expression studies in various tissues.