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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

Updated: May 18, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

Published on: December 2, 2009

Noncoder: a web interface for exon array-based detection of long non-coding RNAs.

Pascal Gellert1, Yuliya Ponomareva, Thomas Braun

  • 1Max Planck Institute for Heart and Lung Research, Ludwigstr 43, 61231 Bad Nauheim, Germany.

Nucleic Acids Research
|September 27, 2012
PubMed
Summary
This summary is machine-generated.

This study shows how to use exon arrays to profile long non-coding RNA (lncRNA) expression. A new tool, noncoder, enables researchers to analyze lncRNA data efficiently.

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Related Experiment Videos

Last Updated: May 18, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

Published on: December 2, 2009

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Recent advances have identified numerous long non-coding RNAs (lncRNAs) in mammals.
  • The functions of most lncRNAs remain largely uncharacterized despite known tissue-specific and disease-associated expression patterns.

Purpose of the Study:

  • To demonstrate the utility of re-annotated exon array probes for profiling lncRNA expression.
  • To develop a user-friendly web interface, 'noncoder', for analyzing lncRNA expression from exon array data.

Main Methods:

  • Re-annotation of Affymetrix GeneChip Exon 1.0 ST Array probes.
  • Development of the 'noncoder' web interface for data normalization and differential expression analysis.
  • Utilizing CEL files from exon arrays for lncRNA expression profiling.

Main Results:

  • Successfully profiled expression of tens of thousands of lncRNAs using re-annotated exon array probes.
  • Noncoder facilitates detailed inspection of lncRNAs and their isoforms.
  • Identified differentially expressed lncRNAs through user-friendly analysis.

Conclusions:

  • Re-annotated exon arrays are a viable method for lncRNA expression profiling.
  • The noncoder tool provides accessible analysis of lncRNA expression for the research community.
  • Enables efficient identification of lncRNAs for further functional studies.