Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...

Arboviral Encephalitis

Arboviral Encephalitis

Arboviral encephalitis refers to brain inflammation caused by arthropod-borne viruses, particularly those transmitted through mosquito vectors. Among these, West Nile virus (WNV), a member of the Flaviviridae family, is a significant public health concern. WNV is an enveloped, positive-sense, single-stranded RNA virus. Human infection typically begins when an infected mosquito introduces the virus into the dermis during feeding. The primary transmission cycle involves birds as amplifying hosts...

Hepatitis

Hepatitis

Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver. The...

Receptor-mediated Endocytosis

Receptor-mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...

Viruses of Archaea

Viruses of Archaea

Archaeal viruses play a crucial role in the ecosystems of extremophilic archaea, particularly those belonging to the phyla Euryarchaeota and Crenarchaeota. By shaping host evolution and facilitating gene transfer, these viruses influence microbial communities and contribute to genetic diversity in extreme environments. The archaea they infect thrive in acidic hot springs and hydrothermal vents characterized by high temperatures and low pH. Archaeal viruses exhibit remarkable structural...

Intracellular Movement of Viruses and Bacteria

Intracellular Movement of Viruses and Bacteria

Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a virus that...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Evaluation of commercial rapid tests for fast and on-site detection of high-pathogenicity avian influenza H5 virus in poultry.

Microbiology spectrum·2025

Same author

Changes in bone marrow fat fraction and immune cell counts in women with cervical cancer treated with primary chemoradiotherapy.

Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology·2025

Same author

Nanoparticle display of neuraminidase elicits enhanced antibody responses and protection against influenza A virus challenge.

NPJ vaccines·2024

Same author

Dosimetric impact of bone marrow sparing for robustly optimized IMPT for locally advanced cervical cancer.

Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology·2024

Same author

Coronavirus M Protein Trafficking in Epithelial Cells Utilizes a Myosin Vb Splice Variant and Rab10.

Cells·2024

Same author

Ten-year results of the PORTEC-2 trial for high-intermediate risk endometrial carcinoma: improving patient selection for adjuvant therapy.

British journal of cancer·2018

Search research articles

Related Experiment Video

Updated: May 18, 2026

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

Heparan sulfate facilitates Rift Valley fever virus entry into the cell.

S M de Boer¹, J Kortekaas, C A M de Haan

¹Department of Infectious Diseases & Immunology, Virology Division, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.

Journal of Virology

|September 28, 2012

Summary

Rift Valley fever virus (RVFV) requires heparan sulfate, a molecule on cell surfaces, for effective entry into host cells. This finding is crucial for understanding RVFV infection and developing antiviral strategies.

More Related Videos

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Published on: March 2, 2016

Related Experiment Videos

Last Updated: May 18, 2026

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Published on: March 2, 2016

Area of Science:

Virology
Cell Biology
Biochemistry

Background:

Rift Valley fever virus (RVFV) is an emerging arthropod-borne pathogen with broad host and cell tropism.
Understanding the molecular mechanisms of RVFV entry is critical for controlling its spread.

Purpose of the Study:

To investigate the role of cell surface molecules in RVFV entry.
To identify specific host factors required for efficient RVFV infection.

Main Methods:

Virus neutralization assays using heparin.
Enzymatic removal of cell surface heparan sulfate.
Analysis of RVFV entry in genetically modified cells lacking heparan sulfate synthesis.

Main Results:

Heparan sulfate, a glycosaminoglycan, is essential for efficient RVFV entry.
Preincubation with heparin significantly reduced viral entry.
Enzymatic removal or genetic deficiency of heparan sulfate markedly impaired RVFV infection.

Conclusions:

Heparan sulfate acts as a key host cell receptor or co-receptor for RVFV.
Targeting heparan sulfate interactions could be a potential therapeutic strategy against RVFV.