Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional levelĀ in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Platelet parameters and platelet Toll-like receptor 4 (TLR4) expression in patients with sepsis, and the effect of a joint treatment-plan integrating traditional Chinese and western medicine: a clinical study].

Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixueĀ·2011
Same author

A novel kernel Fisher discriminant analysis: constructing informative kernel by decision tree ensemble for metabolomics data analysis.

Analytica chimica actaĀ·2011
Same author

Anterior debridement and reconstruction via thoracoscopy-assisted mini-open approach for the treatment of thoracic spinal tuberculosis: minimum 5-year follow-up.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research SocietyĀ·2011
Same author

[A family-based association study of FXYD6 gene polymorphisms and schizophrenia].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical geneticsĀ·2011
Same author

Prenatal diagnosis of penoscrotal transposition with 2- and 3-dimensional ultrasonography.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in MedicineĀ·2011
Same author

Differentiation of Ī±- or Ī²-aspartic isomers in the heptapeptides by the fragments of [Mā€‰+ā€‰Na]+ using ion trap tandem mass spectrometry.

Journal of the American Society for Mass SpectrometryĀ·2011
Same journal

[Advances in research on neuroelectrophysiological characteristics of post-stroke cognitive impairment based on quantitative electroencephalography and acupuncture interventions].

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhiĀ·2026
Same journal

[Mechanisms and applications of magnesium ion-regulated stem cell functions in promoting tendon-bone interface healing].

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhiĀ·2026
Same journal

[Applications and challenges of ultra-high molecular weight polyethylene fibers in minimally invasive medical devices].

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhiĀ·2026
Same journal

[Research on auditory neurofeedback technology and its multi-disciplinary applications].

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhiĀ·2026
Same journal

[Application and perspective of novel auditory intervention paradigms based on verbal and nonverbal stimuli for severe traumatic brain injury].

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhiĀ·2026
Same journal

[Research progress on the neuromodulation targets in stroke rehabilitation].

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhiĀ·2026
See all related articles

Related Experiment Video

Updated: May 18, 2026

Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae
08:36

Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae

Published on: March 25, 2015

[Research on cationic vector-mediated RNAi].

Xu Zhang1, Huiqin Ding, Bing Wang

  • 1College of Life Science, Liaoning Normal University, Dalian 116029, China.

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi = Journal of Biomedical Engineering = Shengwu Yixue Gongchengxue Zazhi
|September 29, 2012
PubMed
Summary
This summary is machine-generated.

Cationic liposomes efficiently deliver small interfering RNA (siRNA) for gene silencing. Optimized conditions enable effective target gene silencing with low siRNA concentrations, showing minimal impact on cell viability.

More Related Videos

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
08:29

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

DNA Vector-based RNA Interference to Study Gene Function in Cancer
13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

Related Experiment Videos

Last Updated: May 18, 2026

Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae
08:36

Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae

Published on: March 25, 2015

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
08:29

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

DNA Vector-based RNA Interference to Study Gene Function in Cancer
13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Cell Biology

Context:

  • Gene silencing is a crucial tool in molecular biology research and therapeutic development.
  • Cationic liposomes are widely investigated as non-viral vectors for nucleic acid delivery.
  • Efficient and safe delivery of small interfering RNA (siRNA) remains a challenge.

Purpose:

  • To evaluate the efficiency of cationic liposome-mediated small interfering RNA (siRNA) transfer for gene silencing.
  • To determine the optimal conditions for siRNA delivery using cationic liposomes in Hep-2 cells.
  • To assess the impact of siRNA concentration on gene silencing efficacy and cell viability.

Summary:

  • Luciferase siRNA was used to test gene silencing in Hep-2 cells stably expressing luciferase.
  • Cationic liposome (Lipofectamine 2000) demonstrated efficient pDNA transfection and strong siRNA binding capacity.
  • Effective luciferase gene silencing was achieved with low siRNA concentrations, with minimal impact on cell viability at optimal levels.

Impact:

  • This study highlights the potential of optimized cationic liposome formulations for efficient siRNA-mediated gene silencing.
  • The findings contribute to the development of safer and more effective gene therapy strategies.
  • Optimized delivery systems can reduce the required siRNA dose, potentially minimizing off-target effects and improving therapeutic outcomes.