Regional variations in fascicle curvatures within a muscle belly change during contraction

During muscle contraction, the fascicles curve in response to changes in internal pressures within the muscle. Muscle modelling studies have predicted that fascicles curve to different extents in different regions of the muscle and, as such, curvature is expected to vary along and across the muscle belly. In the present study, the local variations in fascicle curvature within the muscle belly were investigated for a range of contractile conditions. B-mode ultrasound scans of the medial and lateral gastrocnemii muscles were collected at five ankle positions-ranging from dorsiflexion to plantarflexion. An automated algorithm was applied to the images in order to extract the local curvatures from the muscle belly regions. Significant variations in fascicle curvature were seen in the superficial-to-deep direction. Curvatures were positive in the superficial layer, negative in the deep layer, and had intermediate values close to zero in the central muscle region. This is indicative of the fascicles following an S-shaped trajectory across the muscle image. The relation between external pressure and curvature regionalization was also investigated by applying elastic compression bandages on the calf. The application of pressure was associated with greater negative curvatures in the distal and central regions of the middle layer, but appeared to have little effect on the superficial and deep layers. The results from this study showed that (1) fascicle curvature increases with contraction level, (2) there is curvature regionalization within the muscle belly, (3) curvature increases with pressure, and (4) fascicles follow an S-shaped trajectory across the muscle images.