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[Optimum extraction technology of total triterpenoids from Hypodematium sinense]
Hua-Li Zhang1, Jun Zhu, Gui-Hua Gao
1School of Pharmacy, Jining Medical University, Rizhao 276826, China. hualizhang@163.com
Objective:
To optimize the extraction technology of total triterpenoids from Hypodematium sinense.
Methods:
With 5% vanillin-glacial acetic acid solution and 72% sulfuric acid as chromogenic agent and the content of total tripenoids as index,using single factor experiment and orthogonal test,the optimal extraction condition was determined.
Results:
The optimal conditions were solid-liquid ratio 1:12, 60% ethanol concentration, and ultrasonic extraction time of 60 min at 60 degrees C.
Conclusion:
The extraction technology is feasible and can be used as extraction process of total triterpenoids from Hypodematium sinense.