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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Updated: May 18, 2026

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

Fast microtomography using bright monochromatic x-rays.

J W Jung1, J S Lee, N Kwon

  • 1X-ray Imaging Center, Pohang University of Science and Technology, San 31, Hyoja-dong, Pohang 790-784, South Korea.

The Review of Scientific Instruments
|October 2, 2012
PubMed
Summary
This summary is machine-generated.

A new microtomography system achieves high-resolution, high-speed imaging using advanced X-ray technology. This breakthrough enables rapid visualization of dynamic processes, such as rising bubbles in liquids.

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Last Updated: May 18, 2026

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Area of Science:

  • Physics
  • Engineering
  • Materials Science

Background:

  • Microtomography is crucial for non-destructive 3D imaging.
  • High-speed imaging is needed to capture dynamic phenomena.
  • Current systems often face limitations in speed or resolution.

Purpose of the Study:

  • To develop a microtomography system capable of high-resolution and high-speed imaging.
  • To demonstrate the system's feasibility for visualizing dynamic processes.
  • To achieve rapid data acquisition for complex phenomena.

Main Methods:

  • Utilized bright monochromatic X-rays at the SPring-8 BL29XU beamline.
  • Integrated a fast-rotating sample stage for rapid data collection.
  • Employed a high-performance X-ray imaging detector.

Main Results:

  • Achieved a shortest scan time of 0.25 seconds with a 1.25 μm effective pixel size.
  • Successfully visualized rising bubbles in a viscous liquid, demonstrating multiphase flow physics.
  • Obtained high spatial resolution (300 nm feature size) and very high temporal resolution (9.8 μs) in radiographs.

Conclusions:

  • The developed microtomography system offers unprecedented speed and resolution.
  • It is a powerful tool for studying dynamic processes in various scientific fields.
  • Enables new possibilities in materials science, fluid dynamics, and beyond.