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Electrofusion of oriented Schizosaccharomyces pombe cells through apical protoplast-protuberances
V Vondrejs1, I Pavlícek, M Kothera
1Department of Biotechnology, Faculty of Science, Charles University, Prague, Czechoslovakia.
Biochemical and Biophysical Research Communications
|January 15, 1990
Abstract:
The electrofusion of oriented Schizosaccharomyces pombe cells through apical protoplast-protuberances was demonstrated. The protuberances arose after an exposure of early-exponential phase cells to digestive enzymes from hepatopancreas of Helix pomatia. The orientation of cylindric cells within pearl chains was produced by the application of inhomogenous alternating electric fields.