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Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers
Published on: December 6, 2019
SAP97 promotes the stability of Nax channels at the plasma membrane
Masahito Matsumoto1, Akihiro Fujikawa, Ryoko Suzuki
1Division of Molecular Neurobiology, National Institute for Basic Biology, Okazaki, Aichi, Japan.
Abstract:
Na(x) is a sodium-level sensor for body fluids expressed in the circumventricular organs in the brain. Na(x) has a putative PSD-95/Disc-large/ZO-1 (PDZ)-binding motif at the carboxyl (C)-terminus. Here we found that several PDZ proteins bind to Na(x) by PDZ-array overlay assay. Among them, synapse-associated protein 97 (SAP97/DLG1) was coexpressed with Na(x) in the subfornical organ. In C6 glioblastoma cells, destruction of the PDZ-binding motif of Na(x) or depletion of SAP97 resulted in a decrease in cell-surface Na(x), which was attenuated with inhibitors of endocytosis. These results indicate that SAP97 contributes to the stabilization of Na(x) channels at the plasma membrane.
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