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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads
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DNA detection based on fluorogenic nanospheres.

Xin Shu1, Yonghui Liu, Jin Zhu

  • 1Department of Polymer Science and Engineering, School of Chemistry and Chemical Engineering, State Key Laboratory of Coordination Chemistry, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing, 210093, China.

Angewandte Chemie (International Ed. in English)
|October 2, 2012
PubMed
Summary
This summary is machine-generated.

Fluorogenic nanospheres conjugated to DNA enable highly sensitive detection of target DNA. Addition of N-butylmorpholine triggers nanosphere dissolution, releasing fluorophores for intense blue fluorescence at hybridization sites.

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Sensitive detection of specific DNA sequences is crucial for diagnostics and research.
  • Existing methods may face limitations in sensitivity or require complex amplification steps.

Purpose of the Study:

  • To develop a novel, highly sensitive, and selective method for DNA detection using fluorogenic nanospheres.
  • To achieve signal amplification spatially separated from the hybridization event.

Main Methods:

  • Conjugation of fluorogenic nanospheres to DNA probes.
  • Hybridization of DNA probes to target DNA sequences.
  • Triggered dissolution of nanospheres using N-butylmorpholine to release fluorophores.

Main Results:

  • Demonstrated intense blue fluorescence emission localized at DNA hybridization sites.
  • Achieved high sensitivity with detection limits as low as 100 zmol.
  • Exhibited high selectivity for target DNA sequences.

Conclusions:

  • The developed 'high and dry' fluorescence method offers a sensitive and selective approach for DNA detection.
  • Separating hybridization and signal amplification enhances performance.
  • This technique has potential applications in molecular diagnostics and biological sensing.