Related Experiment Video
Updated: May 18, 2026
11:41
Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells
Published on: December 16, 2018
Isolation and characterization of canine amniotic membrane-derived multipotent stem cells
Sang-Bum Park1, Min-Soo Seo, Hyung-Sik Kim
1Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Plos One
|October 2, 2012
Summary
Canine amniotic membrane tissue yields multipotent stem cells (MSCs). These canine amniotic membrane-derived multipotent stem cells (cAM-MSCs) show potential for veterinary regenerative medicine and cell therapy applications.
Area of Science:
- Veterinary Medicine
- Stem Cell Biology
- Regenerative Medicine
Background:
- Human amniotic membrane is a known source of stem cells with therapeutic applications.
- The potential of canine amniotic membrane as a stem cell source is underexplored.
Purpose of the Study:
- To isolate and characterize multipotent stem cells from canine amniotic membrane tissue.
- To evaluate the potential of these cells for veterinary regenerative medicine.
Main Methods:
- Isolation and culture of canine amniotic membrane-derived multipotent stem cells (cAM-MSCs) in DMEM.
- Characterization of cAM-MSCs using flow cytometry for immunophenotype and cumulative population doubling level (CPDL) for proliferation.
- Assessment of trilineage differentiation potential under specific culture conditions.
Main Results:
- Successfully isolated and cultured cAM-MSCs with fibroblast-like morphology adhering to plastic.
- Confirmed multipotency of cAM-MSCs through trilineage differentiation studies.
- Demonstrated cAM-MSCs as a viable stem cell source in dogs.
Conclusions:
- Canine amniotic membrane is a rich source of multipotent stem cells (cAM-MSCs).
- cAM-MSCs exhibit characteristics suitable for cell therapy.
- These cells hold promise for applications in veterinary regenerative medicine.
