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Related Experiment Video

Updated: May 18, 2026

Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope (AFM-SECM)

Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope (AFM-SECM)

Published on: February 10, 2021

Alternating current scanning electrochemical microscopy with simultaneous fast-scan cyclic voltammetry.

Jason A Koch¹, Melinda B Baur, Erica L Woodall

¹Department of Chemistry, Illinois State University, Normal, Illinois 61790-4160, United States.

Analytical Chemistry

|October 3, 2012

Summary

This summary is machine-generated.

This study introduces a novel technique combining fast-scan cyclic voltammetry (FSCV) and alternating current scanning electrochemical microscopy (AC-SECM) for simultaneous chemical and topographical imaging. This method enables detailed analysis of substrates, including living cells, without redox mediators.

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Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation

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Last Updated: May 18, 2026

Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope (AFM-SECM)

Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope (AFM-SECM)

Published on: February 10, 2021

Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation

Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation

Published on: October 10, 2018

Area of Science:

Electrochemistry
Scanning Probe Microscopy
Chemical Imaging

Background:

Simultaneous chemical and topographical imaging is crucial for understanding complex biological and material systems.
Existing techniques often require multiple probes or lack the resolution for detailed cellular analysis.

Purpose of the Study:

To develop and validate a combined fast-scan cyclic voltammetry (FSCV) and alternating current scanning electrochemical microscopy (AC-SECM) technique.
To enable simultaneous measurement of impedance and faradaic current using a single probe.
To demonstrate the technique's utility in imaging living cell cultures.

Main Methods:

Integration of a high-frequency, low-amplitude sine wave with FSCV waveforms (10-1000 V s(-1)).
Utilized lock-in amplifier and analog circuit for AC signal amplitude measurement.
Employed a single carbon fiber probe for simultaneous electrochemical and topographical data acquisition.

Main Results:

The combined FSCV and AC-SECM technique provided simultaneous chemical and topographical information.
The added AC signal exhibited negligible effects on the voltammetric measurements at a carbon fiber electrode.
Successfully imaged cellular respiration and topography in living cell cultures without redox mediators.

Conclusions:

The developed FSCV and AC-SECM combination offers a powerful tool for multidimensional chemical and topographical imaging.
This technique eliminates the need for redox mediators, simplifying sample preparation and analysis.
The approach holds significant promise for advanced imaging applications in biological and material sciences.