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Updated: May 18, 2026
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Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
Published on: July 22, 2015
Viewing dynamic interactions of proteins and a model lipid membrane with atomic force microscopy
Anthony S Quinn1, Jacob H Rand, Xiao-Xuan Wu
1Department of Pathology and Microscopy Imaging Center, College of Medicine, University of Vermont, Burlington, VT, USA. asquinn@zoo.uvm.edu
Methods in Molecular Biology (Clifton, N.J.)
|October 3, 2012
Summary
This chapter details a versatile membrane preparation and atomic force microscopy (AFM) imaging technique for lipid studies. The protocol ensures high-quality data acquisition for researchers using AFM instruments.
Area of Science:
- Biophysics
- Materials Science
Background:
- Atomic Force Microscopy (AFM) is crucial for visualizing molecular structures.
- Standardized protocols are needed for reproducible lipid membrane studies using AFM.
Purpose of the Study:
- To present a generalized membrane preparation and AFM dynamic imaging protocol.
- To enable successful application across various lipid studies and AFM instruments.
- To guide novice researchers in acquiring high-quality AFM images.
Main Methods:
- A homogenous membrane preparation technique.
- A dynamic imaging procedure compatible with Asylum Research MFP-3D BIO and Bruker BioScope AFM systems.
- Procedures supplemented by ellipsometry, western blotting, and dot-blots for molecular component verification.
Main Results:
- The protocol is adaptable to multiple lipid studies and AFM setups.
- Detailed procedures for sample preparation and MFP-3D BIO operation are provided.
- Novice personnel can acquire quality images with minimal supervision.
Conclusions:
- The presented protocol facilitates reproducible membrane imaging using AFM.
- High purity and activity of molecular components are essential for successful imaging.
- This guide enhances the use of AFM for lipid research and analysis.
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