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Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle
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Published on: March 31, 2013

Functional redundancy between Cdc14 phosphatases in zebrafish ciliogenesis.

Aurélie Clément1, Lilianna Solnica-Krezel, Kathleen L Gould

  • 1Howard Hughes Medical Institute and Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
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Zebrafish Cdc14A and Cdc14B phosphatases have overlapping roles in ciliogenesis, crucial for development. Depleting either protein causes developmental defects, highlighting their shared function in cilia formation.

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Molecular Genetics

Background:

  • Cyclin-dependent kinases (Cdks) and phosphatases regulate cell cycle progression.
  • Cdc14 phosphatases are vital for cell cycle exit and cytokinesis in yeast.
  • Vertebrate CDC14A and CDC14B are linked to DNA repair, meiosis, centrosome duplication, and ciliogenesis.

Purpose of the Study:

  • To investigate potential functional compensation between CDC14A and CDC14B paralogs.
  • To elucidate the roles of CDC14A and CDC14B in zebrafish development, particularly in ciliogenesis.

Main Methods:

  • Utilized antisense morpholino oligonucleotides to inhibit Cdc14A1 function in zebrafish embryos.
  • Observed and analyzed developmental phenotypes, including body curvature and left-right asymmetry.
  • Assessed the role of Cdc14A1 and Cdc14B in ciliogenesis within the Kupffer's vesicle (KV) and other ciliated tissues.

Main Results:

  • Cdc14A1 depletion in zebrafish embryos resulted in ventrally curved bodies and left-right asymmetry, mirroring Cdc14B deficiency.
  • Both Cdc14A1 and Cdc14B were found to be essential for ciliogenesis in the KV and other ciliated tissues, independent of cell cycle functions.
  • Reciprocal rescue experiments demonstrated that cdc14b RNA could rescue KV cilia length defects in Cdc14A1-deficient embryos, and vice versa for cdc14a1 RNA in Cdc14B-deficient embryos.

Conclusions:

  • This study provides the first evidence of overlapping functions between Cdc14A and Cdc14B in zebrafish development.
  • Cdc14A and Cdc14B play complementary roles in the critical process of ciliogenesis.