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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

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Modulation of Golgi-associated microtubule nucleation throughout the cell cycle

Ana Rita Ramada Maia1, Xiaodong Zhu, Paul Miller

  • 1Department of Cell and Developmental Biology, Vanderbilt University Medical Center, 465 21st Avenue South, Nashville, TN 37232, USA.

Cytoskeleton (Hoboken, N.J.)
|October 3, 2012
PubMed

Insights

Golgi-derived microtubules form throughout the cell cycle, but their nucleation is reduced during mitosis. These microtubules are essential for Golgi reassembly during telophase.

Area of Science:

  • Cell Biology
  • Cytoskeleton Dynamics
  • Organelle Biology

Background:

  • Microtubules (MTs) form a significant part of the cellular network in interphase cells.
  • A subpopulation of MTs originates from the Golgi membrane.

Purpose of the Study:

  • To investigate if the Golgi membrane retains its ability to nucleate MTs at different cell cycle stages.
  • To understand the role of Golgi-derived MTs in cell division and Golgi reassembly.

Main Methods:

  • Live cell imaging
  • Immunofluorescence microscopy

Main Results:

  • Golgi-derived MTs were observed at multiple cell cycle stages, including G(1), G(2), and mitosis.
  • The capacity of Golgi to nucleate MTs is significantly down-regulated during mitosis compared to interphase.
  • Golgi-derived MTs are crucial for efficient Golgi assembly during telophase.

Conclusions:

  • Golgi's MT nucleation capacity is cell cycle-regulated.
  • Noncentrosomal MTs originating from the Golgi may have specific functions beyond telophase Golgi assembly.

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